Lieven

Matrix metalloproteinase-17 (MMP-17), also known as MT4-MMP, is a membrane-anchored member of the MMP family. It plays an important role in extracellular matrix remodeling, cell migration, and cellular response to inflammatory stimuli. This article discusses a practical ELISA-based workflow for monitoring MMP-17 protein expression under inflammatory conditions using in vitro cytokine stimulation models. This approach […]

Introduction: The Role of ssDNA in Modern NGS As next-generation sequencing (NGS) evolves to accommodate low-input, degraded, or chemically modified DNA, the demand for single-stranded DNA (ssDNA) library preparation has grown significantly. While traditional double-stranded DNA (dsDNA) protocols remain effective for high-quality, abundant DNA, they fall short in contexts where only trace amounts of fragmented

Introduction In the context of livestock management and pet health surveillance, rapid diagnostic tools serve as indispensable assets for identifying potential infectious threats at the animal-human interface. Among the most crucial areas of monitoring are zoonotic pathogens—infectious agents naturally shared between animals and people. These microorganisms, which include bacteria, protozoa, and certain parasites, can spread

Magnetic bead-based technologies have become foundational in molecular biology laboratories, facilitating high-throughput and reproducible extraction of nucleic acids with minimal manual handling. This approach enables researchers to isolate high-quality DNA and RNA from a variety of biological matrices—such as blood, tissue, cultured cells, and microbial sources—without hazardous chemicals or centrifugation. This article presents a detailed,

Abstract The ability to quantify intracellular kinase activity within three-dimensional (3D) culture platforms is critical for advancing physiological relevance in in vitro models. Focal adhesion kinase 1 (FAK1), a cytoplasmic tyrosine kinase central to mechanotransduction, is involved in diverse cellular processes including adhesion, migration, and differentiation. This article presents a comprehensive technical overview of integrating

Introduction: Why Kinome Profiling Needs Better ELISA Integration Kinome profiling aims to assess the global activity of protein kinases within cells and tissues, offering a comprehensive view of how cells respond to signals. In cattle biology, this approach becomes essential to decode how signaling changes affect health, growth, and productivity. A significant bottleneck in kinome

Abstract Phytase enzymes catalyze the hydrolysis of phytic acid, a storage form of phosphorus found in plant seeds. This study compares microbial-derived phytase and plant-derived phytase using in vitro assay kits to quantify enzyme kinetics, pH and thermal profiles, and phytate substrate affinity. These insights are essential for feed formulation, enzymology research, and agronomic residue

Alkaline Phosphatase (ALP) is a non-specific phosphomonoesterase widely distributed in mammalian tissues such as liver, bone, kidney, and placenta. It plays a key role in the hydrolysis of phosphate esters under alkaline conditions, liberating inorganic phosphate. The Alkaline Phosphatase Assay Kit is a fundamental tool used in biochemical research, cellular biology, enzyme kinetics, and molecular

The Quantitative DNA Relaxation Assay is one of the most important biochemical assays used in molecular biology to evaluate the activity of human DNA topoisomerase I. This enzyme plays a critical role in maintaining DNA topology during replication, transcription, and other cellular processes. In this article, we explore in depth how to optimize detection conditions

Introduction to DNA Topology and Relaxation DNA in living organisms is not just a passive genetic material but a dynamic, topologically regulated molecule. In prokaryotic cells such as Escherichia coli, DNA can exist in supercoiled, relaxed, or linear forms. These conformations are influenced by environmental conditions and internal enzymatic activity. DNA topological states impact replication