Lieven

Introduction In the context of livestock management and pet health surveillance, rapid diagnostic tools serve as indispensable assets for identifying potential infectious threats at the animal-human interface. Among the most crucial areas of monitoring are zoonotic pathogens—infectious agents naturally shared between animals and people. These microorganisms, which include bacteria, protozoa, and certain parasites, can spread […]

Magnetic bead-based technologies have become foundational in molecular biology laboratories, facilitating high-throughput and reproducible extraction of nucleic acids with minimal manual handling. This approach enables researchers to isolate high-quality DNA and RNA from a variety of biological matrices—such as blood, tissue, cultured cells, and microbial sources—without hazardous chemicals or centrifugation. This article presents a detailed,

Abstract The ability to quantify intracellular kinase activity within three-dimensional (3D) culture platforms is critical for advancing physiological relevance in in vitro models. Focal adhesion kinase 1 (FAK1), a cytoplasmic tyrosine kinase central to mechanotransduction, is involved in diverse cellular processes including adhesion, migration, and differentiation. This article presents a comprehensive technical overview of integrating

Introduction: Why Kinome Profiling Needs Better ELISA Integration Kinome profiling aims to assess the global activity of protein kinases within cells and tissues, offering a comprehensive view of how cells respond to signals. In cattle biology, this approach becomes essential to decode how signaling changes affect health, growth, and productivity. A significant bottleneck in kinome

Abstract Phytase enzymes catalyze the hydrolysis of phytic acid, a storage form of phosphorus found in plant seeds. This study compares microbial-derived phytase and plant-derived phytase using in vitro assay kits to quantify enzyme kinetics, pH and thermal profiles, and phytate substrate affinity. These insights are essential for feed formulation, enzymology research, and agronomic residue

Alkaline Phosphatase (ALP) is a non-specific phosphomonoesterase widely distributed in mammalian tissues such as liver, bone, kidney, and placenta. It plays a key role in the hydrolysis of phosphate esters under alkaline conditions, liberating inorganic phosphate. The Alkaline Phosphatase Assay Kit is a fundamental tool used in biochemical research, cellular biology, enzyme kinetics, and molecular

The Quantitative DNA Relaxation Assay is one of the most important biochemical assays used in molecular biology to evaluate the activity of human DNA topoisomerase I. This enzyme plays a critical role in maintaining DNA topology during replication, transcription, and other cellular processes. In this article, we explore in depth how to optimize detection conditions

Introduction to DNA Topology and Relaxation DNA in living organisms is not just a passive genetic material but a dynamic, topologically regulated molecule. In prokaryotic cells such as Escherichia coli, DNA can exist in supercoiled, relaxed, or linear forms. These conformations are influenced by environmental conditions and internal enzymatic activity. DNA topological states impact replication

The comet assay, or single-cell gel electrophoresis (SCGE), is an established genotoxicity technique used extensively in molecular biology and cell-based testing environments. It is one of the most sensitive and reproducible methods for detecting DNA strand breaks at the single-cell level. The assay’s versatility enables its adaptation into two main protocols: alkaline comet assay and

Overview Alkaline phosphatase (ALP) assays are frequently used in molecular research to measure enzymatic dephosphorylation activity under alkaline conditions. They are essential in a wide range of biological studies where phosphate turnover plays a role in cell signaling, metabolic regulation, or structural changes in biomolecules. Integrating ALP assays into high-throughput screening (HTS) formats enables efficient,